Assessment of Clinical Characteristics and Chest CT Findings of COVID-19 Prediagnosed Patients Admitted to the Emergency Department: A Retrospective Study

Research | DOI: https://doi.org/10.31579/2693-4779/058

Assessment of Clinical Characteristics and Chest CT Findings of COVID-19 Prediagnosed Patients Admitted to the Emergency Department: A Retrospective Study

  • Murat Muratoglu* 1
  • Sevil Ozek 1
  • Hayrettin Meftun Kaptan 1
  • Ishaaq Sakwa Eshikumo 1
  • Ishaaq Sakwa Eshikumo 1
  • 1 Baskent University Faculty of Medicine, Department of Emergency, Ankara, Turkey.

*Corresponding Author: Murat Muratoglu, Baskent University Faculty of Medicine, Department of Emergency Fevzi Cakmak Street Number: 45 06490 Bahcelievler, Ankara, Turkey.

Citation: M Muratoglu, S Ozek, HM Kaptan, IS Eshikumo, E Biyikli. (2021) Assessment of Clinical Characteristics and Chest CT Findings of COVID-19 Prediagnosed Patients Admitted to the Emergency Department: A Retrospective Study. Clinical Research and Clinical Trials. 4(2); DOI: 10.31579/2693-4779/058

Copyright: © 2021 Murat Muratoglu, This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: 06 August 2021 | Accepted: 14 August 2021 | Published: 24 August 2021

Keywords: atrial fibrillation; CT; pneumonia; clinic features; COVID-19

Abstract

Introduction: This study aimed to define the clinical features and laboratory findings that may be associated with COVID-19 pneumonia in patients with suspected COVID-19 pneumonia who presented to the emergency department in order to be able to recognize the disease early until molecular tests are available, and thus to isolate sick people as soon as possible.

Patients and Methods: We retrospectively examined patients who were evaluated with the suspicion of COVID-19 between March 2020 and August 2020. We used the patient information management system to record patient demographic and clinical features, laboratory findings, and CT results. In subgroups with and without pneumonia, we compared these characteristics in confirmed COVID-19 patients.

Results: Our study included 1,277 patients who were evaluated for suspected COVID-19. COVID-19 pneumonia was detected in 71.2% (n=210) of a total of 295 (23.4%) patients with a diagnosis of COVID-19 who were confirmed by RT-PCR positivity. Although 56.2% (n=18) of those with pneumonia were men, no statistically significant relationship was detected between gender distribution and CT findings (p=0.449). When we compared the patients in 3 subgroups according to age groups, it was observed that 59.4% (n=38) of those with negative CT findings were in the 18-44 age group, and 44.8% (n=94) of those with positive CT findings were 65 and over age group, and this distribution was found to be statistically significant (p<0.001). Pneumonia was detected in 58.3% (n=49) of COVID-19 cases (n=84) with a suspected exposure history (p=0.008). The most common symptoms in patients with positive CT findings (n=210) were fever (40.5% n=85) and fatigue (40.5% n=85), cough (32.9% n=69), and shortness of breath (28.6% n=60). The most common comorbidity in cases diagnosed with COVID-19 was HT (33.9%; n=100/295), followed by AF (19.3%; n=57/295), and the rates of HT and AF in those with COVID-19 pneumonia were statistically significantly higher compared to the CT-negative group (p<0.001 and p=0.002, respectively).COVID-19 pneumonia was more common in hospitalized patients (n=124), and hospitalization lengths were longer in both emergency and ICU admissions (p<0.001 and p=0.008). In our analysis in terms of the survival relationship, it was observed that 87.5% (n=35) of the COVID-19 cases who died had pneumonia (p<0.042). Low saturation and high respiratory rate per minute at the time of admission were associated with COVID-19 pneumonia (p<0.001). In the group with COVID-19 pneumonia, haemoglobin, GFR, and pCO2 values were recorded lower at the time of admission compared to patients with negative CT (p<0.001, p<0.001, p<0.05, respectively), while leukocytes, neutrophils, CRP, D-dimer, LDH, hs-troponin, AST and bilirubin were recorded as higher (p<0.05, p<0.01, p<0.001, p<0.001, p<0.001, p<0.001, p=0.01, p=0.01, respectively).

Conclusion: Identifying patient features linked with COVID-19 pneumonia at the time of admission may influence our decision to have CT scans in emergency departments, regardless of the RT-PCR result, and may result in early disease identification and treatment.

Introduction

The causative agent of COVID-19, which turned into a pandemic in a short time after it emerged in Wuhan, China, in December 2019, has been identified as SARS-CoV-2, and it can create a picture of critical illness that can range from a simple upper respiratory tract infection to severe respiratory and organ failure [1-3]. Vaccination campaigns are currently underway over the world, but the disease has not yet been eradicated [4]. As a result, the battle against COVID-19 continues. Although case fatality rates are modest compared to SARS and MERS outbreaks, earlier studies have demonstrated that the rate of spread of SARS-CoV-2 is higher than other coronavirus epidemics in the last two decades [5]. The accumulation of the first cases in those who came into contact with the Wuhan South China Seafood City Market in the south of Wuhan suggested that the causative agent was a zoonotic coronavirus [6]. However, soon the reporting of imported cases and the increase in the epidemic rate [7] were shown as evidence of human-to-human transmission [8], and it was understood that this transmission occurred [6-10] by droplet route, contact with the surfaces of the secretions of infected individuals, and possible faecal-oral transmission [9, 10]. Given this information, being able to diagnose sick individuals early plays a crucial role in bringing the disease under control by breaking the chain of contact [11].  The high false-negative rate of RT-PCR results, which is the gold-standard method in the definitive diagnosis of COVID-19, problems in sample transport, lack of kits in every hospital, problems experienced during sample collection, and changing host dynamics (immune response, additional diseases, age, gender, etc.) affecting viral load make the early diagnosis of the disease difficult, and it makes it challenging to isolate sick individuals from healthy individuals in the early period, notably in crowded hospitals where patient admissions are high and logistically insufficient to provide contact isolation, during periods when cases accumulate and in epidemic regions [12, 13]. Particularly in COVID-19, where the contagiousness is so high, many diagnostic tools have been tried to be developed to fill the diagnostic gaps created by RT-PCR while also benefiting from the synergistic effect with molecular tests, and thorax imaging has come to the fore in this regard [13, 14]. Thoracic CT seems to be superior to chest radiography [16], especially because infected individuals are asymptomatic, can show symptoms even in the early stages of the disease, have high sensitivity [15], and parenchymal features of COVID-19 pneumonia. Although its usage alone is not encouraged, it has been shown to have benefits when combined with epidemiological, clinical, laboratory, and molecular tests, such as the ability to detect disease early in highly suspect cases and providing information about the disease's stage and prognosis [17, 18]. However, there appears to be no clear consensus in clinical practice regarding which clinical features should be prioritized for imaging in COVID-19 patients [19]. Furthermore, if we can recognize the epidemiological, clinical, and laboratory features possibly associated with pneumonia, we can avoid overusing CT as a diagnostic tool, protect patients from the risks of CT, such as radiation and a contact chain, and develop a more cost-effective health economics strategy [20, 21]. For these reasons, in our study, we sought to define clinical features and laboratory findings that may be associated with COVID-19 pneumonia in order to recognize the disease early in the period before molecular tests are available in suspected COVID-19 patients who presented to the emergency department, allowing us to isolate sick individuals as soon as possible and initiate treatment process.

Patients and Methods

After receiving approval from our faculty's Ethics Committee, we retrospectively analysed patients who presented to the Baskent University Emergency Medicine Clinic Emergency Service with COVID-19 symptoms and met the inclusion criteria between March 2020 and August 2020. We planned our study in accordance with the principles of the "World Medical Association Declaration of Helsinki". We determined the inclusion criteria to include all patients aged 18 years and older who were evaluated in our emergency department with a pre-diagnosis of COVID-19. The entire sample of our study consisted of 1,277 patients who met the inclusion criteria. We retrospectively analysed the demographic characteristics of the patients at admission, their comorbidities, their complaints at the time of admission, the time they elapsed until they applied to the emergency department, laboratory results, thorax computed tomography findings, PCR swab test results, the length of stay if hospitalization occurred, the epicrisis of the department, whether they needed non-invasive/invasive mechanical ventilation, whether they received oxygen therapy, their morbidity and mortality information, and the file notes recorded in the emergency department through the patient information management system. We recorded all times in day format and determined the primary outcome variable as 30-day mortality. In this study, we examined the patients' CT findings over their documented CT report records and classified them as positive if CT findings of COVID-19 pneumonia were reported and negative if they were not. We identified confirmed COVID-19 patients based on the positivity of RT-PCR swab samples. We categorized the entire sample into three subgroups: those with a final diagnosis of COVID-19 if the RT-PCR test is positive, those with a non-COVID-19 diagnosis if their RT-PCR test is negative and another diagnosis can explain their current status, and those whose final diagnosis is uncertain (unable to be diagnosed) if RT-PCR test is negative, but there is no additional differential diagnosis to explain the clinic and COVID-19 is excluded. CT scans were performed with Siemens 'SOMATOM go. All' branded CT devices with 32 detectors were used in the imaging of patients with suspected COVID-19 in the Radiology department, and RT-PCR test analyses were performed with the 'Rotor-Gene Q' brand RT-PCR device.

Statistical Analysis

Frequency (n) and percentage (%) values were used in the evaluation of categorical variables as descriptive statistics in the study. Compliance of numerical variables with normal distribution was examined by Kolmogorov-Smirnov test of normality, and median (minimum-maximum) values were given as descriptive statistics because numerical variables were not suitable for normal distribution. The Mann-Whitney U test and the Kruskal-Wallis test were used to examine whether there was a statistical difference between the distributions of numerical variables. In testing the dependency between categorical variables, Pearson Chi-Square Test results were obtained when test assumptions were met, and Fisher-Exact test results were obtained when not. Type I error probability was determined as α=0.05 in all hypothesis tests, and the SPSS v22.0 package program was used for statistical evaluations.

Results

In our study, which included 1,277 patients who were evaluated with a preliminary diagnosis of COVID-19, CT findings were found to be positive in 34.9% (n=396) of 1,136 patients who underwent thoracic CT. 53.3% (n=211) of those with positive CT findings were male, and there was no statistically significant relationship between gender distribution and CT findings (p=0.088). Those with positive CT findings were found to be older than those with negative CT findings (p<0 n=305) n=740) n=217) n=396) n=54/396) n=106/740) p=0.744). p=0.002).The n=396) n=137), n=124), n=120), n=109), p=0.003 p=0.009). n=156] n=37]) n=69] n=20]) n=202) n=269) p=0.011). p=0.012), n=244) n=176], n=77) n=18)>

Table 1. Clinical characteristics of patients who underwent CT in the whole sample.

U=Mann-Whitney U test,      x2= Pearson's Chi Square test

As in the whole sample, we compared CT findings with patient clinical features in confirmed COVID-19 cases (Table 2). COVID-19 pneumonia was detected in 71.2% (n=210) of a total of 295 (23.4%) patients with a diagnosis of COVID-19 who were confirmed by RT-PCR positivity. While 56.2% (n=118/210) of those with pneumonia were male, no statistically significant correlation was found between gender distribution and CT findings (p=0.449). In COVID-19 patients, the mean age was 55.5±19.3 years in males and 52.7±21.3 years in females (p=0.261). It was observed that those with positive CT findings were older than those with negative CT findings (p<0 n=38/64) n=94/210) n=49/84) p=0.008). p=0.030).The n=85/210) n=85/210), n=69/210), n=60/210), n=30/34) n=34/295), n=60/70) n=70/295), n=85/132) n=132/295), n=25/44) n=44/295), n=41/73) n=10/20) n=20/295)>0.05). When the time from the onset of symptoms to admittance to the emergency room was compared, there was no significant difference between the groups (3 (0-14) in the pneumonia group, 3 (0-7) in the CT-negative group; p=0.318). As in the whole sample, the most common comorbidity in cases diagnosed with COVID-19 was HT (33.9%; n=100/295), followed by AF (19.3%; n=57/295), and the rates of HT and AF were statistically significantly higher in those with COVID-19 pneumonia compared to the CT-negative group (p<0 p=0.002, n=26), n=21) p=0.102 p=0.221). n=124), p=0.008).92.7% n=115) n=9) p=0.172).>0.05). In our analysis in terms of the survival relationship, it was seen that 87.5% (n=35) of the COVID-19 cases who died had pneumonia (p<0 n=23) n=13)>0.05).

Table 2. CT findings and clinical features in COVID-19 cases.

*Kruskal-Wallis test    F= Fisher-Exact test    x2= Pearson's Chi Square test

The analysis results of the relationship between pneumonia and vital parameters and laboratory findings in COVID-19 patients are presented in Table 3. Low saturation and high respiratory rate per minute at the time of admission were associated with COVID-19 pneumonia. In the group with COVID-19 pneumonia, haemoglobin, GFR, and pCO2 values were recorded lower at the time of admission compared to patients with negative CT, while leukocytes, neutrophils, CRP, D-dimer, LDH, hs-troponin, AST, and bilirubin were recorded as higher (Table 3).

Table 3. Comparison of the distribution of CT findings, vital parameters and laboratory values in COVID-19 patients.

Test statistics: Mann-Whitney U test

*thd/mcl: Thousand / Microliter

Discussion

The epidemiological, clinical, and laboratory features that may be associated with the presence of pneumonia on thorax CT in suspected and confirmed COVID-19 cases were investigated in this study. We discovered that COVID-19 pneumonia patients were older and had worse outcomes, that the prevalence of underlying comorbidities was higher, that a history of suspected exposure was associated with pneumonia, and that active smoking was more common in COVID-19 patients without pneumonia.

In parallel with previous studies [22], the fact that patients with COVID-19 pneumonia were older in our study may be related to the fact that SARS-CoV-2 causes more extensive alveolar damage and more inflammatory exudation in elderly patients due to fundamental reasons such as the fact that underlying comorbidities are more common than the younger population, immune response blunting with age, and changing pulmonary physiology [23, 24]. 

Even though the male sex ratio was found to be higher in patients with COVID-19 pneumonia, we did not detect a statistically significant difference in gender distribution in our study. Similar to our results, Li et al. found that advanced age and the frequency of comorbidity were associated with the severity of the disease, but they reported that they did not detect a significant difference in terms of gender [25]. At this point, we diverged from Chen et al.'s study in which they examined the characteristics of 99 patients with COVID-19 pneumonia and found male gender, advanced age, and comorbidity to be associated with pneumonia, and from Palaiodimos et al.'s studies in which male gender was reported as an independent risk factor for poor in-hospital outcomes and mortality [26,27]. Similarly, advanced age and comorbidities were found to be connected with the disease and poor outcomes in Wang et al.'s study of 138 hospitalized patients with COVID-19 pneumonia, but no relationship between gender and prognosis could be detected [28]. The literature has suggested that men are more prone to SARS-CoV-2 infection due to the fact that ACE2 receptor expression is higher in men due to the change of gonadal organs towards testicles, women have more immune-modulatory genes due to the higher number of X chromosomes, women are more careful about hygienic issues and men are more involved in social life [29, 30]. In our study, we think that the fact that male gender was not associated with COVID-19 pneumonia, patients in the pneumonia group were older; therefore, the immune response was decreased in both sexes at an advanced age, regardless of gender, and they were equally susceptible to the disease with geriatric physiological changes is related to the fact that the high socio-cultural level of the region where our hospital is located changes the environmental factors on gender.

Although the data related to smoking and COVID-19 pneumonia have not been clarified yet, there are opinions that smoking predisposes to the disease by impairing mucociliary activity, increasing ACE2 receptor expression, and hand-mouth contact [31, 32]. On the other hand, there are studies in which the relationship between smoking and COVID-19 could not be demonstrated either at the receptor level or clinically and prognostically [32, 33]. In short, more research is needed on the relationship between smoking and COVID-19. The results of our study data show an inverse relationship between smoking and the frequency of pneumonia, but it should not be concluded that smoking protects from COVID-19 pneumonia. Our results may be related to the older age of the group with COVID-19 pneumonia and the decrease in the frequency of smoking in the older age group.

The most common symptoms in patients with COVID-19 pneumonia were recorded as fever, fatigue, cough, and dyspnoea in our study. In this respect, our results were in line with the literature data [34, 35]. Fever, dyspnoea, headache, sore throat, myalgia, and nausea/vomiting at presentation were associated with those with COVID-19 pneumonia compared to COVID-19 cases without pneumonia. We discovered that, in addition to the respiratory and systemic symptoms of viral pneumonia, several patients with COVID-19 pneumonia had gastrointestinal symptoms as their initial symptom and that nausea and vomiting were related to the frequency of pneumonia in COVID-19 patients in our study; and these findings may be related to the fact that the virus uses ACE2 receptors during its entry into the host cell and that ACE2 receptors are located in most of our body systems, showing a wide distribution not only in respiratory system cells but also in the gastrointestinal system, hepatobiliary system, cardiovascular system, and central nervous system [36, 37]. This pathogenesis is of a nature that explains the organ tropism of SARS-CoV-2. 

We found that the most common comorbidities were HT and AF, both in the whole sample and in the COVID-19 cases, and both were observed more frequently in the group with COVID-19 pneumonia. In the light of literature, we know that the existence of comorbidities (such as pre-existing chronic conditions, especially hypertension, cardiovascular disease, and diabetes) are highly associated with catching COVID-19, and the disease progresses more severely in those with comorbidities [38-41]. In a meta-analysis of 6,560 patients investigating the relationship between HT and poor outcomes in patients with COVID-19 pneumonia, the presence of HT was found to be associated with mortality, disease severity, ICU rates, ARDS risk, and progression; however, this association was reported to be more assertive in sub-studies with the lower male gender [42]. While HT is associated with COVID-19 pneumonia, although mortality in this group is higher in percentage, a statistical relationship between mortality rates and HT could not be demonstrated in our study. We believe that there is a need for more comprehensive studies investigating other patient factors that will affect the relationship between mortality and COVID-19.

In most studies to date, AF has not been mentioned as a predictor or risk factor for COVID-19 disease but as an arrhythmic complication caused by the disease [43-45]. It has not been clearly demonstrated whether the presence of AF is associated with poor outcomes in patients, but it has been reported that newly developed AF during the course of COVID-19 disease worsens the results by increasing the risk of thromboembolism [46]. Regarding the frequency of AF in COVID-19 patients, AF has been reported to be among the top 10 comorbidities seen in COVID-19, seventh, according to the New York State Department of Health data [47]. Similarly, AF was observed in 22% of COVID-19 patients who died of COVID-19, according to Italian data [48]. AF was the second most common condition in COVID-19 patients in our study, and it was strongly linked to patients who died from COVID-19 pneumonia. These results may be related to the increased ACE2 receptor expression in AF patients, making AF patients more susceptible to SARS-CoV-2 infection [49].

Similar to the literature in our study, pneumonia was associated with more hospitalizations; the need for hospitalization was higher in patients with COVID-19 than those without pneumonia, and the length of hospitalization was longer. Presence of pneumonia was also associated with the need for respiratory support. However, in our study, it was seen that HFNO and mechanical ventilation (both NIMV and IMV) were applied at lower percentages compared to previous studies [50] in patients with COVID-19 pneumonia.  We believe this is owing to the fact that during the pandemic, patients with critical COVID-19 pneumonia were referred to external centres due to our hospital's COVID-19 ICU's inadequate capacity and so were excluded from the study. Patients with COVID-19 pneumonia had lower saturation values at admission and higher respiratory rates per minute, as expected and consistent with literature data [25].

In our study, we found that haemoglobin, GFR, and pCO2 values at the time of admission were lower, leukocytes, neutrophils, CRP, D-dimer, LDH, hs-troponin, AST, and bilirubin values were higher in the group with COVID-19 pneumonia compared to CT negative COVID-19 patients. Previous studies have revealed that acute phase reactants are elevated in COVID-19 patients due to the activation of pro-inflammatory pathways in SARS-CoV-2 infection, as in all viral and bacterial infections [51]. We believe that our findings, which show that lymphocytopenia is not associated with COVID-19 pneumonia, contrary to the literature, are due to the fact that the patients are in the early stages of their disease, between admission and onset of symptoms, and thus we are not yet dealing with a cytokine storm that will cause lymphocyte apoptosis [52]. Moreover, the low pCO2 values at the time of admission in patients may be related to the high respiratory rate per minute at the time of admission and the early respiratory alkalosis.

Based on the negative CT findings in some of the patients with positive RT-PCR test in our study, our results also support the literature information that CT imaging alone will not be sufficient to exclude the disease without RT-PCR test and that molecular tests should be used for definitive diagnosis in highly suspicious cases and epidemic regions [53, 54].

Our study has some limitations. First, it is unclear what criteria clinicians use in the emergency department to identify patients as highly suspected cases of COVID-19; clinical gestalt was at the forefront in this regard, which may have contributed to our study's low rate of confirmed/suspected COVID-19 cases. Second, since it is unclear which classification system radiologists define as “positive” for CT findings, a study in which CT findings are reassessed, and a methodology plan in which individual distributions of findings are analysed may positively affect the results. Finally, the fact that most patients with critical pneumonia were referred to external centres due to the lack of COVID-19 ICU capacity in our hospital may have affected the distribution of critically ill patients in our sample group and, therefore, the outcomes of pneumonia cases.

In conclusion; advanced age, presence of pre-existing comorbidities, fever and dyspnoea, myalgia, sore throat, headache or nausea-vomiting at the time of admission, presence of a history of exposure, low saturation and high respiratory rate at the time of admission, and high acute phase reactants are associated with COVID-19 pneumonia, and being able to identify these patient characteristics at the time of admission may affect our decision for CT scan regardless of the RT-PCR result and may result in early diagnosis and treatment of the disease.

Funding

None declared.

Conflict of interest

None declared. 

References

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Dr Tong Ming Liu

I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.

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Husain Taha Radhi

I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.

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S Munshi

Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.

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Tania Munoz

“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.

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George Varvatsoulias

Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.

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Rui Tao

Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.

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Khurram Arshad